Journal: Frontiers in Plant Science

Article Title: Genetic and morphological variants of Acidovorax avenae subsp. avenae cause red stripe of sugarcane in China

doi: 10.3389/fpls.2023.1127928

Figure Lengend Snippet: In silico analysis of RFLP patterns of 17 strains of Acidovorax avenae subsp. avenae from sugarcane in China based on the internal transcribed spacer (ITS) region of 16S-23S rDNA.

Article Snippet: A restriction fragment length polymorphism (RFLP) analysis of the Aaa sequences obtained with primers RS-ITS-F1/RS-ITS-R1 was performed in silico using restriction enzymes Hin dIII and Eco RI with DNAMAN software (Lynnon Biosoft, San Ramon, USA).

Techniques: In Silico, Sequencing

Journal: The Journal of Neuroscience

Article Title: Thrombomodulin as a New Marker of Lesion-Induced Astrogliosis: Involvement of Thrombin through the G-Protein-Coupled Protease-Activated Receptor-1

doi: 10.1523/JNEUROSCI.20-07-02543.2000

Figure Lengend Snippet: Thrombomodulin mRNA is upregulated after lesion of AMV. In situ hybridization of thrombomodulin mRNA after lesion of the AMV. AMV were taken at different times after lesioning and hybridized with a digoxigenin-labeled riboprobe, followed by immunodetection with an anti-digoxigenin-alkaline phosphatase antibody revealed by a diformazan precipitate. Times after lesion:A, 0 hpl; B, 9 hpl; C, 1 dpl; D, F, 2 dpl; E, 6 dpl. The lesion is marked by asterisks inA–E, except in F in which it is out of the field on the right. Note that, at 2 dpl inF, there is a clear limit between thrombomodulin mRNA-positive (right) and mRNA-negative (left) cells, which might mark the limit of a front of astrocytosis (see Discussion). Scale bars, 100 μm.

Article Snippet: The following items were supplied by Boehringer Mannheim (Mannheim, Germany): digoxigenin detection kit, sheep anti-digoxigenin-alkaline phosphatase Fab fragments, blocking reagent, digoxigenin in vitro transcription kit, Eco RI and Hin dIII enzymes, and proteinase K. The sodium lauryl sarcosinate (Sarkosyl) was from Fluka Chemie AG (Buchs, Switzerland).

Techniques: In Situ Hybridization, Labeling, Immunodetection

Journal: BioMed Research International

Article Title: Expression and Immunogenicity of Two Recombinant Fusion Proteins Comprising Foot-and-Mouth Disease Virus Structural Protein VP1 and DC-SIGN-Binding Glycoproteins

doi: 10.1155/2017/7658970

Figure Lengend Snippet: Design of the recombinant transfer vector for the VP1 protein and the VP1-gp120 and VP1-E2 fusion proteins and confirmation of the recombinant transfer vector and baculovirus bacmids. M indicates the DNA size marker. (a) Sites of insertion of the coding sequences for the VP1 protein and the VP1-gp120 and VP1-E2 fusion proteins in the baculovirus transfer vector pFastBac 1. (b) The recombinant transfer vectors pFastBac-VP1, pFastBac-VP1-gp120, and pFastBac-VP1-E2 were digested with the restriction endonucleases Bam HI and Hin dIII and subjected to agarose gel electrophoresis. Lanes 1, 3, and 5: undigested plasmids. Lanes 2, 4, and 6: pFastBac-VP1, pFastBac-VP1-gp120, and pFastBac-VP1-E2 digested with Bam HI and Hin dIII, respectively. (c) The recombinant bacmids rBacmid-VP1, rBacmid-VP1-gp120, and rBacmid-VP1-E2 were amplified by PCR with the M13 universal primers, and the PCR products were subjected to agarose gel electrophoresis. Lanes 1, 2, and 3: PCR products from rBacmid-VP1, rBacmid-VP1-gp120, and rBacmid-VP1-E2, respectively.

Article Snippet: Similarly, the restriction enzyme sites Bam HI and Hin dIII and a His tag were introduced in the fusion sequence and synthesized by Sangon Biotech (Shanghai, China).

Techniques: Recombinant, Plasmid Preparation, Marker, Agarose Gel Electrophoresis, Amplification